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Biosecurity Queensland has quarantined two adjoining properties in the Boondall area after test results from a deceased horse returned a positive result for Hendra virus infection.
Queensland Chief Veterinary Officer, Dr Rick Symons said the horse was euthanased yesterday.
"We will now be testing six more horses at this site," he said.
"These horses will be monitored daily over the next month."
The Boondall case follows confirmation today that a horse euthanased at a Hervey Bay property on Wednesday also had Hendra virus infection.
In Queensland, there are now six separate locations where Biosecurity Queensland is managing Hendra virus cases - Beaudesert, Mt Alford, Park Ridge, Kuranda, Hervey Bay and Boondall.
Details on where people in the Hervey Bay and Boondall areas will be able to access public information about Hendra virus will be finalised shortly.
Up-to-date information on Hendra virus is available at www.biosecurity.qld.gov.au, including important workplace health and safety information for horse properties and other horse-related businesses.
For more information, contact Biosecurity Queensland on 13 25 23 or visit www.biosecurity.qld.gov.au